Sept 22

 Today we made 2 concave slides so that we would be able to look at a drop of both our environmental and unknown bacteria that had been growing in broth since September 20th.

Broth

Concave slide: environmental sample

Also, a video of the environmental sample....LOOK AT THEM GO!!!(Rod Shaped)

Concave slide: Unknown sample (Circular Shaped)

To make a concave slide one must add a drop of Vaseline to the four corners of a cover slip. Then, using a sterile inoculating loop, take a sample of contaminated broth and place a drop of the broth in the center of the covers slip. Then with the concave slide - making sure you are indeed using the concave side- place the concave side on top of the cover slip making sure the drop of broth is in the center of the concave section of the slide. The Vaseline sticks to the slide so that the drop of broth stays intact.

Below is a picture of the mobility test for our unknown bacteria. As you can see the bacteria only grew within the area where the inoculating needle punctured the semisolid agar.

We also looked at the stained slides we made last class

Stained Environmental sample (Rod shaped)

Stained unknown sample (Circular Shaped)